Interaction Details


Details of experiments between Q9NYB5 and Q9UPQ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.76
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.8
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 11.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.71
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: Biotin-ado-ado-NYWPGKETQL

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.73
Normalized holdup BI: 0.85
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.6
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.61
Normalized holdup BI: 0.76
Immobilized partner concentration (10-6M): 11.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.59
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.3
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.99
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL

Protein B

Protein: PDZRN3
Fragment: 405-515
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.09
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835