Interaction Details


Details of experiments between Q9NUP9 and Q8TAA9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV
Average holdup BI: 0.08
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.13
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.94
PUBMED ID: 36115835