Interaction Details


Details of experiments between Q9NUP9 and Q8N448
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: LNX2
Fragment: 681-690
Site: PBM
Sequence: TVICWPGSLV
Construct: Biotin-Ahx-TVICWPGSLV
Average holdup BI: 0.4
Normalized holdup BI: 0.6
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.98
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.