Interaction Details


Details of experiments between Q9NUP9 and P17386
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV31-E6
Fragment: 140-149
Site: PBM
Sequence: SRRPRTETQV
Construct: biotin-ttds-SRRPRTETQV
Average holdup BI: 0.08
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.13
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 3.93
PUBMED ID: 36115835