Interaction Details


Details of experiments between Q9NUP9 and O75912
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LIN7C
Fragment: 1-197
Construct: endogenous protein (Jurkat)

Protein B

Protein: DGKI
Fragment: 1056-1065
Site: PBM
Sequence: IGHEDLETAV
Construct: biotin-ttds-IGHEDLETAV
Average holdup BI: 0.56
Immobilized partner concentration (10-6M): 10
P value: 6.5
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.1
PUBMED ID: 37942667
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKI
Fragment: 1056-1065
Site: PBM
Sequence: IGHEDLETAV
Construct: biotin-ado-ado-IGHEDLETAV
Average holdup BI: 0.34
Normalized holdup BI: 0.38
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.57
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.