Interaction Details


Details of experiments between Q9NSK0 and Q04917
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KLC4
Fragment: 549-558
Site: 14-3-3_pSer554
Modification: (SEP)554
Sequence: LQRSGSLGKI
Construct: LQRSG(pSer)LGKI (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.49
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 130
P value: 6.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.87
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: KLC4
Fragment: 561-570
Site: 14-3-3_pSer566
Modification: (SEP)566
Sequence: VLRRSSELLV
Construct: VLRRS(pSer)ELLV (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.17
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 130
P value: 2.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: KLC4
Fragment: 585-594
Site: 14-3-3_pSer590
Modification: (SEP)590
Sequence: MKRAASLNYL
Construct: MKRAA(pSer)LNYL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.86
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 130
P value: 7.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))