Interaction Details


Details of experiments between Q9NS75 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.67
Normalized holdup BI: 0.99
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 7.14
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.76
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.93
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.83
Normalized holdup BI: 0.93
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.78
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.8
Normalized holdup BI: 0.88
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.72
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.75
Normalized holdup BI: 0.9
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.57
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.57
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.48
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.61
Normalized holdup BI: 0.72
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.22
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.65
Normalized holdup BI: 0.77
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.14
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.39
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.1
PUBMED ID: 36115835