Interaction Details


Details of experiments between Q9NS75 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.86
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.99
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 6.55
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.8
Normalized holdup BI: 0.94
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 6.08
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.73
Normalized holdup BI: 0.91
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.83
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.64
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.64
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 2.51
Standard deviation of dissociation constant (10-6M): 0.13
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.6
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.52
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.66
Holdup BI standard deviation: 0
Normalized holdup BI: 0.85
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.38
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.65
Normalized holdup BI: 0.77
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.34
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 9

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.56
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.67
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.92
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.71
PUBMED ID: 36115835