Interaction Details


Details of experiments between Q9NR12 and Q9UDY2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDLIM7
Fragment: 448-457
Site: PBM
Sequence: LCKSHAFSHV
Construct: Biotin-Ahx-LCKSHAFSHV

Protein B

Protein: TJP2
Fragment: 24-128
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Normalized holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.09
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: PDLIM7
Fragment: 1-85
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TJP2
Fragment: 1181-1190
Site: PBM
Sequence: QSARYRDTEL
Construct: biotin-ttds-QSARYRDTEL
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835