Interaction Details


Details of experiments between Q9IR59 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.54
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.54
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 6
Measured pKd: 4.67
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 26.85
Standard deviation of dissociation constant (10-6M): 4.74
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.57
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.48
PUBMED ID: 36115835