Interaction Details


Details of experiments between Q9IR59 and Q00013
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV

Protein B

Protein: MPP1
Fragment: 69-153
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.79
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.75
PUBMED ID: 36115835