Interaction Details


Details of experiments between Q9HD26 and Q9NS75
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GOPC
Fragment: 278-373
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV
Average holdup BI: 0.34
Normalized holdup BI: 0.36
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.54
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: GOPC
Fragment: 278-373
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.29
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GOPC
Fragment: 278-373
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV
Average holdup BI: 0.23
Holdup BI standard deviation: 0
Normalized holdup BI: 0.32
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.27
PUBMED ID: 36115835