Interaction Details


Details of experiments between Q9HCD6 and Q9NY99
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TANC2
Fragment: 1981-1990
Site: PBM
Sequence: PKRPFVESNV
Construct: biotin-ttds-PKRPFVESNV

Protein B

Protein: SNTG2
Fragment: 70-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.29
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.37
PUBMED ID: 36115835