Interaction Details


Details of experiments between Q9HCD6 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TANC2
Fragment: 1981-1990
Site: PBM
Sequence: PKRPFVESNV
Construct: biotin-ttds-PKRPFVESNV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.37
Normalized holdup BI: 0.42
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: TANC2
Fragment: 1981-1990
Site: PBM
Sequence: PKRPFVESNV
Construct: biotin-ttds-PKRPFVESNV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Normalized holdup BI: 0.26
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.31
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: TANC2
Fragment: 1981-1990
Site: PBM
Sequence: PKRPFVESNV
Construct: biotin-ttds-PKRPFVESNV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.77
PUBMED ID: 36115835