Interaction Details


Details of experiments between Q9H8Y8 and P26554
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GORASP2
Fragment: 1-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.37
Normalized holdup BI: 0.46
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GORASP2
Fragment: 1-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835