Interaction Details


Details of experiments between Q9H5P4 and Q14721
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNB1
Fragment: 849-858
Site: PBM
Sequence: AHGSTRDQSI
Construct: biotin-ado-ado-AHGSTRDQSI
Average holdup BI: 0.07
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.67
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.