Interaction Details


Details of experiments between Q9H5P4 and Q13224
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZD7
Fragment: 858-953
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIN2B
Fragment: 1475-1484
Site: PBM
Sequence: EKLSSIESDV
Construct: Biotin-Ahx-EKLSSIESDV
Average holdup BI: 0.08
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.77
PUBMED ID: 36115835