Interaction Details


Details of experiments between Q9H0B6 and P27348
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KLC2
Fragment: 540-549
Site: 14-3-3_pSer545
Modification: (SEP)545
Sequence: LRRSGSFGKL
Construct: LRRSG(pSer)FGKL (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 130
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: KLC2
Fragment: 577-586
Site: 14-3-3_pSer582
Modification: (SEP)582
Sequence: MKRASSLNFL
Construct: MKRAS(pSer)LNFL (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.82
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 130
P value: 6.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.54
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: KLC2
Fragment: 584-593
Site: 14-3-3_pSer589
Modification: (SEP)589
Sequence: NFLNKSVEEP
Construct: NFLNK(pSer)VEEP (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.63
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 130
P value: 2.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.11
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: KLC2
Fragment: 603-612
Site: 14-3-3_pSer608
Modification: (SEP)608
Sequence: DSRTLSSSSM
Construct: DSRTL(pSer)SSSM (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.11
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 130
P value: 3.47
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: KLC2
Fragment: 605-614
Site: 14-3-3_pSer610
Modification: (SEP)610
Sequence: RTLSSSSMDL
Construct: RTLSS(pSer)SMDL (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.26
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 3.91
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.43
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: KLC2
Fragment: 610-619
Site: 14-3-3_pSer615
Modification: (SEP)615
Sequence: SSMDLSRRSS
Construct: SSMDL(pSer)RRSS (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 130
P value: 0.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: KLC2
Fragment: 614-623
Site: 14-3-3_pSer619
Modification: (SEP)619
Sequence: LSRRSSLVG-
Construct: DLSRRS(pSer)LVG (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.11
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 130
P value: 1.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))