Interaction Details


Details of experiments between Q9C0E4 and Q9ULB1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRIP2
Fragment: 934-1023
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV
Average holdup BI: 0.37
Normalized holdup BI: 0.37
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.55
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GRIP2
Fragment: 649-740
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.91
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GRIP2
Fragment: 238-339
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: GRIP2
Fragment: 446-548
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: GRIP2
Fragment: 544-642
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835