Interaction Details


Details of experiments between Q9C0A0 and Q86UT5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CNTNAP4
Fragment: 1299-1308
Site: PBM
Sequence: VNENQKEYFF
Construct: Biotin-Ahx-VNENQKEYFF

Protein B

Protein: NHERF4
Fragment: 464-550
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Normalized holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.1
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: CNTNAP4
Fragment: 1299-1308
Site: PBM
Sequence: VNENQKEYFF
Construct: Biotin-Ahx-VNENQKEYFF

Protein B

Protein: NHERF4
Fragment: 106-208
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.51
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: CNTNAP4
Fragment: 1299-1308
Site: PBM
Sequence: VNENQKEYFF
Construct: Biotin-Ahx-VNENQKEYFF

Protein B

Protein: NHERF4
Fragment: 220-307
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835