Interaction Details


Details of experiments between Q9BZF3 and P62258
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: OSBPL6
Fragment: 285-294
Site: 14-3-3_pSer290
Modification: (SEP)290
Sequence: LQRTQSAPNF
Construct: LQRTQ(pSer)APNF (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.81
Holdup BI standard deviation: 0.32
Immobilized partner concentration (10-6M): 116
P value: 2.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.56
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: OSBPL6
Fragment: 285-294
Site: 14-3-3_pSer290
Modification: (SEP)290
Sequence: LQRTQSAPNF
Construct: LQRTQ(pSer)APNF (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.83
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 143
P value: 7.61
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.55
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: OSBPL6
Fragment: 39-48
Site: 14-3-3_pSer44
Modification: (SEP)44
Sequence: LERTASSSTE
Construct: LERTA(pSer)SSTE (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.19
Holdup BI standard deviation: 0.23
Immobilized partner concentration (10-6M): 116
P value: 0.73
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: OSBPL6
Fragment: 39-48
Site: 14-3-3_pSer44
Modification: (SEP)44
Sequence: LERTASSSTE
Construct: LERTA(pSer)SSTE (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: -0.3
Holdup BI standard deviation: 0.49
Immobilized partner concentration (10-6M): 143
P value: 0.69
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))