Interaction Details


Details of experiments between Q9BYB0 and Q9Q6P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SHANK3
Fragment: 562-670
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.39
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 3
Measured pKd: 4.59
PUBMED ID: 36115835