Interaction Details

Details of experiments between Q9BY41 and Q86XP1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: HDAC8
Fragment: 1-377
Construct: endogenous protein (Jurkat)

Protein B

Protein: DGKH
Fragment: 1211-1220
Site: PBM
Sequence: LGRSTPQSEV
Construct: biotin-ttds-LGRSTPQSEV

Average holdup BI: 0.1

Immobilized partner concentration (10^-6M): 10

P value: 0.16

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Number of measurements: 3

The affinity was below the detection threshold of the assay.

PUBMED ID: 37942667

Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))