Interaction Details

Details of experiments between Q9BXL7 and Q81964
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: CARD11
Fragment: 660-756
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Average holdup BI: -0.06

Holdup BI standard deviation: 0.03

Normalized holdup BI: -0.06

Normalized holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 13.7

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835