Interaction Details


Details of experiments between Q9BX40 and NC_000001.11
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LSM14B
Fragment: 1-385
Construct: endogenous protein (K562)

Protein B

Protein: 4945_HAP
Fragment: 203681644-203681669
Construct: Biotin-TCAGCCCTCCGTTTTGTCACCTACAC
Average holdup BI: 0.52
Immobilized partner concentration (10-6M): 5
P value: 5.18
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.34
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: LSM14B
Fragment: 1-385
Construct: endogenous protein (K562)

Protein B

Protein: 51_HAP
Fragment: 203681832-203681862
Construct: Biotin-GAATTGAGAGGTATCTTACCGCTCCCACTCC
Average holdup BI: 0.48
Immobilized partner concentration (10-6M): 5
P value: 5.88
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.27
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 3

Protein A

Protein: LSM14B
Fragment: 1-385
Construct: endogenous protein (K562)

Protein B

Protein: 4945_WT
Fragment: 203681644-203681669
Modification: rs10751449/rs10736845
Construct: Biotin-TCAGCCCTCCGTATCGTCACCTACAC
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 5
P value: 6.12
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.29
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 4

Protein A

Protein: LSM14B
Fragment: 1-385
Construct: endogenous protein (K562)

Protein B

Protein: 51_WT
Fragment: 203681832-203681862
Modification: rs10751451
Construct: Biotin-GAATTGAGAGGTATCTTATCGCTCCCACTCC
Average holdup BI: 0.4
Immobilized partner concentration (10-6M): 5
P value: 4.84
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.13
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))