Interaction Details


Details of experiments between Q9BST9 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RTKN
Fragment: 554-563
Site: PBM
Sequence: QPRTWLQSPV
Construct: Biotin-Ahx-QPRTWLQSPV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.28
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.35
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: RTKN
Fragment: 554-563
Site: PBM
Sequence: QPRTWLQSPV
Construct: Biotin-Ahx-QPRTWLQSPV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Normalized holdup BI: 0.24
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.27
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: RTKN
Fragment: 554-563
Site: PBM
Sequence: QPRTWLQSPV
Construct: Biotin-Ahx-QPRTWLQSPV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Normalized holdup BI: 0.21
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.19
PUBMED ID: 36115835