Interaction Details


Details of experiments between Q9BSE5 and Q96JH8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV

Protein B

Protein: RADIL
Fragment: 973-1075
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.36
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.39
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.59
PUBMED ID: 36115835