Interaction Details


Details of experiments between Q9BSE5 and Q8TBB1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV

Protein B

Protein: LNX1
Fragment: 371-470
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.28
Holdup BI standard deviation: 0
Normalized holdup BI: 0.31
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.42
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV

Protein B

Protein: LNX1
Fragment: 261-371
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Holdup BI standard deviation: 0
Normalized holdup BI: 0.14
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.98
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV

Protein B

Protein: LNX1
Fragment: 500-596
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.61
PUBMED ID: 36115835