Interaction Details


Details of experiments between Q9BSE5 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV

Protein B

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.51
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV

Protein B

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV

Protein B

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835