Interaction Details


Details of experiments between Q9BRQ0 and Q9H223
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PYGO2
Fragment: 57-86
Construct: biotin-ahx-HHSSNWLRNPFKVLDKNQEPALWKQLIK-CONH2

Protein B

Protein: EHD4
Fragment: 1-541
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.51
Immobilized partner concentration (10-6M): 10
P value: 11.24
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-09-20
Number of measurements: 6
Measured pKd: 5.01
Experimental Details

Batch = Helsinki_batch8; Ncontrols = 24; Nsamples = 6; Npreys = 6577; threshold curvature: 1.87