Interaction Details


Details of experiments between Q9BRQ0 and P56282
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PYGO2
Fragment: 1-406
Construct: endogenous proteins (Jurkat)

Protein B

Protein: POLE2
Fragment: 1-527
Construct: avi-his6-MBP-fusion protein
Average holdup BI: 0.37
Immobilized partner concentration (10-6M): 26
P value: 1.36
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-02-02
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch6; Ncontrols = 12; Nsamples = 6; Npreys = 6515; threshold curvature: 2.18

Experiment series 2

Protein A

Protein: PYGO2
Fragment: 57-86
Construct: biotin-ahx-HHSSNWLRNPFKVLDKNQEPALWKQLIK-CONH2

Protein B

Protein: POLE2
Fragment: 1-527
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 10
P value: 2.24
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-09-20
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch8; Ncontrols = 24; Nsamples = 6; Npreys = 6577; threshold curvature: 1.87