Interaction Details


Details of experiments between Q9BRQ0 and O43426
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PYGO2
Fragment: 1-406
Construct: endogenous proteins (Jurkat)

Protein B

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 10
P value: 0.35
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.72

Experiment series 2

Protein A

Protein: PYGO2
Fragment: 1-406
Construct: endogenous proteins (Jurkat)

Protein B

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 10
P value: 0.07
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-12-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54

Experiment series 3

Protein A

Protein: PYGO2
Fragment: 57-86
Construct: biotin-ahx-HHSSNWLRNPFKVLDKNQEPALWKQLIK-CONH2

Protein B

Protein: SYNJ1
Fragment: 1-1573
Construct: endogenous proteins (Jurkat)
Average holdup BI: -0.13
Immobilized partner concentration (10-6M): 10
P value: 0.46
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-09-20
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch8; Ncontrols = 24; Nsamples = 6; Npreys = 6577; threshold curvature: 1.87