Interaction Details


Details of experiments between Q9BQQ3 and P36762
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GORASP1
Fragment: 1-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV49-L2
Fragment: 512-521
Site: PBM
Sequence: RRRKRKRTYL
Construct: Biotin-Ahx-RRRKRKRTYL
Average holdup BI: 0.35
Normalized holdup BI: 0.42
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 36115835