Interaction Details


Details of experiments between Q99700 and P49418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ATXN2
Fragment: 1-1313
Construct: endogenous protein (Jurkat)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.52
Immobilized partner concentration (10-6M): 10
P value: 4.97
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.03
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ATXN2
Fragment: 583-597
Site: PxxP_3
Sequence: RPPSRPPSRPSRPPS
Construct: Bio Ado Ado-RPPSRPPSRPSRPPS-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.6
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.98
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: ATXN2
Fragment: 112-126
Site: PxxP_1
Sequence: ASPGAPPAAPTRASP
Construct: Bio Ado Ado-ASPGAPPAAPTRASP-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: ATXN2
Fragment: 139-153
Site: PxxP_2
Sequence: SLARPAPGCPRPACE
Construct: Bio Ado Ado-SLARPAPGCPRPACE-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: ATXN2
Fragment: 995-1009
Site: PxxP_4
Sequence: KTYRAVPNMPQQRQD
Construct: Bio Ado Ado-KTYRAVPNMPQQRQD-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680