Interaction Details


Details of experiments between Q99501 and P49418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GAS2L1
Fragment: 1-681
Construct: endogenous protein (Jurkat)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.73
Immobilized partner concentration (10-6M): 10
P value: 3.11
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.43
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: GAS2L1
Fragment: 185-199
Site: PxxP_2
Sequence: TETAPAPGTPARGPR
Construct: Bio Ado Ado-TETAPAPGTPARGPR-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.78
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: GAS2L1
Fragment: 644-658
Site: PxxP_6
Sequence: PSRIPTPRGPRRPSG
Construct: Bio Ado Ado-PSRIPTPRGPRRPSG-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.57
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: GAS2L1
Fragment: 166-180
Site: PxxP_1
Sequence: RELRAAPPAPNAPAA
Construct: Bio Ado Ado-RELRAAPPAPNAPAA-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.49
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: GAS2L1
Fragment: 326-340
Site: PxxP_3
Sequence: RSTKEGPETPPRPRD
Construct: Bio Ado Ado-RSTKEGPETPPRPRD-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: GAS2L1
Fragment: 386-400
Site: PxxP_4
Sequence: RLTTGTPASPRRPPA
Construct: Bio Ado Ado-RLTTGTPASPRRPPA-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: GAS2L1
Fragment: 466-480
Site: PxxP_5
Sequence: GSGRSTPQTPRARSP
Construct: Bio Ado Ado-GSGRSTPQTPRARSP-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680