Interaction Details

Details of experiments between Q96T37 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: RBM15
Fragment: 1-977
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.29

Immobilized partner concentration (10^-6M): 10

P value: 6.86

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2022-06-29

Number of measurements: 3

Measured pK_d: 4.61

PUBMED ID: 38995680

Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.11

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

Measured pK_d: 3.85

PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: RBM15
Fragment: 1-977
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.37

Immobilized partner concentration (10^-6M): 10

P value: 2.44

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2022-06-29

Number of measurements: 3

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 4

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: -0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.06

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 3.54

PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: -0.07

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.08

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 3.71

PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: -0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.08

Immobilized partner concentration (10^-6M): 8

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 4.02

PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0

Immobilized partner concentration (10^-6M): 8

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.06

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 3.55

PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: -0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.07

Immobilized partner concentration (10^-6M): 10

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

Measured pK_d: 3.87

PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: -0.01

Immobilized partner concentration (10^-6M): 10

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.09

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

Measured pK_d: 3.73

PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: -0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.08

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

Measured pK_d: 3.68

PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.08

Immobilized partner concentration (10^-6M): 13

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 3.81

PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: -0.03

Immobilized partner concentration (10^-6M): 13

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: RBM15
Fragment: 311-325
Site: PxxP_1
Sequence: ALGRLPPPPPPPLPR
Construct: Bio Ado Ado-ALGRLPPPPPPPLPR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: RBM15
Fragment: 583-597
Site: PxxP_2
Sequence: DSDWVPPPPPVRERS
Construct: Bio Ado Ado-DSDWVPPPPPVRERS-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Average holdup BI: -0.08

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680