Interaction Details


Details of experiments between Q96RT1 and Q68D42
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TMEM215
Fragment: 226-235
Site: PBM
Sequence: QGRWDHETIV
Construct: Biotin-Ahx-QGRWDHETIV
Average holdup BI: 0.16
Holdup BI standard deviation: 0
Normalized holdup BI: 0.18
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.09
PUBMED ID: 36115835