Interaction Details

Details of experiments between Q96RT1 and Q13009
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TIAM1
Fragment: 1582-1591
Site: PBM
Sequence: APSRKLNTEI
Construct: Biotin-Ahx-APSRKLNTEI

Average holdup BI: -0

Holdup BI standard deviation: 0.02

Normalized holdup BI: -0

Normalized holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835