Interaction Details


Details of experiments between Q96RT1 and P06463
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.08
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.12
Holdup BI standard deviation: 0.09
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 13.3
P value: 0
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.01
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.17
Normalized holdup BI: 0.2
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 43
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.78
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.13
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.15
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 43
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 3.61
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.11
Normalized holdup BI: 0.14
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 43
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.58
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: RLQRRRETQV
Construct: Biotin-ttds-RLQRRREpTQV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835