Interaction Details


Details of experiments between Q96RT1 and P04201
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAS1
Fragment: 316-325
Site: PBM
Sequence: CNTVTVETVV
Construct: biotin-ttds-CNTVTVETVV
Average holdup BI: 0.04
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.48
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.