Interaction Details


Details of experiments between Q96RT1 and O75084
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.22
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 6
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.74
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.16
Holdup BI standard deviation: 0
Normalized holdup BI: 0.18
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 21.5
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.04
PUBMED ID: 36115835