Interaction Details


Details of experiments between Q96QZ7 and Q9IR59
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.79
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.89
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.54
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.46
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.02
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAGI1
Fragment: 986-1098
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.37
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI1
Fragment: 638-725
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.15
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.28
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI1
Fragment: 1146-1238
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.21
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAGI1
Fragment: 836-926
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.2
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835