Interaction Details


Details of experiments between Q96QZ7 and Q92793
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI1
Fragment: 986-1098
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.17
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI1
Fragment: 1146-1238
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: 0.01
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAGI1
Fragment: 638-725
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835