Interaction Details


Details of experiments between Q96QZ7 and Q8WWN8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARAP3
Fragment: 1417-1431
Site: Internal_PBM
Construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARAP3
Fragment: 1417-1431
Site: Internal_PBM
Construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835