Interaction Details


Details of experiments between Q96QZ7 and P11166
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC2A1
Fragment: 483-492
Site: PBM
Sequence: FHPLGADSQV
Construct: biotin-ttds-FHPLGADSQV
Average holdup BI: 0.03
Holdup BI standard deviation: 0
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC2A1
Fragment: 483-492
Site: PBM
Sequence: FHPLGADSQV
Construct: biotin-ttds-FHPLGADSQV
Average holdup BI: 0.01
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.01
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835