Interaction Details


Details of experiments between Q96QZ7 and P03409
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI1
Fragment: 986-1098
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.73
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 5.22
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Measured dissociation constant (10-6M): 9.92
Standard deviation of dissociation constant (10-6M): 0.24
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.59
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.94
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI1
Fragment: 638-725
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.49
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.75
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: Biotin-ado-ado-SEKHFRETEV
Average holdup BI: 0.32
Normalized holdup BI: 0.36
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.53
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.25
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.28
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.45
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAGI1
Fragment: 1146-1238
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.32
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.41
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: MAGI1
Fragment: 836-926
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.25
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.28
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: Biotin-ado-ado-SEKHFRETEV
Average holdup BI: -0.06
Normalized holdup BI: -0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 10

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.05
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835