Interaction Details


Details of experiments between Q96QC0 and P00519
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PPP1R10
Fragment: 1-940
Construct: endogenous protein (Jurkat)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 10
P value: 7.34
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.99
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: PPP1R10
Fragment: 712-726
Site: PxxP_4
Sequence: GNEPPPPPPPFRGAR
Construct: Bio Ado Ado-GNEPPPPPPPFRGAR-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.59
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: PPP1R10
Fragment: 498-512
Site: PxxP_1
Sequence: FLNKESPHEPDPEPY
Construct: Bio Ado Ado-FLNKESPHEPDPEPY-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: PPP1R10
Fragment: 663-677
Site: PxxP_2
Sequence: GPRLLGPPPPPRGGD
Construct: Bio Ado Ado-GPRLLGPPPPPRGGD-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: PPP1R10
Fragment: 677-691
Site: PxxP_3
Sequence: DPFWDGPGDPMRGGP
Construct: Bio Ado Ado-DPFWDGPGDPMRGGP-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680