Interaction Details


Details of experiments between Q96Q42 and P61981
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ALS2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: GKKSSSLVDI
Construct: GKKSS(pSer)LVDI (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.61
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 129
P value: 9.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.09
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: ALS2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: GKKSSSLVDI
Construct: GKKSS(pSer)LVDI (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.62
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 8.28
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.08
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: ALS2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: GSRRLSLPGL
Construct: GSRRL(pSer)LPGL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.8
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 129
P value: 4.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.49
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: ALS2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: GSRRLSLPGL
Construct: GSRRL(pSer)LPGL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.62
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 134
P value: 4.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.08
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: ALS2
Fragment: 525-534
Site: 14-3-3_pThr530
Modification: (TPO)530
Sequence: RTEVWTWGKG
Construct: RTEVW(pThr)WGKG (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.87
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 129
P value: 5.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.7
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: ALS2
Fragment: 525-534
Site: 14-3-3_pThr530
Modification: (TPO)530
Sequence: RTEVWTWGKG
Construct: RTEVW(pThr)WGKG (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.85
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 4.91
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.62
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: ALS2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: GKKSSSLVDI
Construct: GKKSS(pSer)LVDI (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.57
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 7.89
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.07
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: ALS2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: GSRRLSLPGL
Construct: GSRRL(pSer)LPGL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.79
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 5.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.52
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: ALS2
Fragment: 525-534
Site: 14-3-3_pThr530
Modification: (TPO)530
Sequence: RTEVWTWGKG
Construct: RTEVW(pThr)WGKG (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.81
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 115
P value: 4.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))