Interaction Details


Details of experiments between Q96Q42 and P62258
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ALS2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: GKKSSSLVDI
Construct: GKKSS(pSer)LVDI (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.25
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 143
P value: 6.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.36
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: ALS2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: GKKSSSLVDI
Construct: GKKSS(pSer)LVDI (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.18
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 116
P value: 4.52
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: ALS2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: GSRRLSLPGL
Construct: GSRRL(pSer)LPGL (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.44
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 143
P value: 2.73
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.74
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: ALS2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: GSRRLSLPGL
Construct: GSRRL(pSer)LPGL (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.13
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 116
P value: 0.8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: ALS2
Fragment: 525-534
Site: 14-3-3_pThr530
Modification: (TPO)530
Sequence: RTEVWTWGKG
Construct: RTEVW(pThr)WGKG (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.68
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 143
P value: 5.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.17
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: ALS2
Fragment: 525-534
Site: 14-3-3_pThr530
Modification: (TPO)530
Sequence: RTEVWTWGKG
Construct: RTEVW(pThr)WGKG (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.55
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 116
P value: 3.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.02
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))