Interaction Details


Details of experiments between Q96Q42 and P31947
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ALS2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: GKKSSSLVDI
Construct: GKKSS(pSer)LVDI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.27
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 4.73
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.51
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: ALS2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: GKKSSSLVDI
Construct: GKKSS(pSer)LVDI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.3
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 142
P value: 5.84
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.49
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: ALS2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: GSRRLSLPGL
Construct: GSRRL(pSer)LPGL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.1
Holdup BI standard deviation: 0.19
Immobilized partner concentration (10-6M): 113
P value: 0.49
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: ALS2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: GSRRLSLPGL
Construct: GSRRL(pSer)LPGL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.14
Holdup BI standard deviation: 0.19
Immobilized partner concentration (10-6M): 142
P value: 0.71
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: ALS2
Fragment: 525-534
Site: 14-3-3_pThr530
Modification: (TPO)530
Sequence: RTEVWTWGKG
Construct: RTEVW(pThr)WGKG (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.48
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 142
P value: 4.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.81
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: ALS2
Fragment: 525-534
Site: 14-3-3_pThr530
Modification: (TPO)530
Sequence: RTEVWTWGKG
Construct: RTEVW(pThr)WGKG (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.31
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 113
P value: 1.96
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.59
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: ALS2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: GKKSSSLVDI
Construct: GKKSS(pSer)LVDI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.24
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 4.49
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.46
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: ALS2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: GSRRLSLPGL
Construct: GSRRL(pSer)LPGL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.28
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 111
P value: 2.37
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.55
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: ALS2
Fragment: 525-534
Site: 14-3-3_pThr530
Modification: (TPO)530
Sequence: RTEVWTWGKG
Construct: RTEVW(pThr)WGKG (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.35
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 111
P value: 2.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))